ID:
publications-2592
Type:
Peer reviewed articles
Year:
2018
Authors:
Laura Clusa, E. GarcĂa-VĂĄzquez
Title:
A simple, rapid method for detecting seven common invasive fish species in Europe from environmental DNA
Venue/Journal:
Aquatic Conservation: Marine and Freshwater Ecosystems
DOI:
10.1002/aqc.2890
Research type:
Simulation & Modeling
Water System:
Uncategorized
Technical Focus:
Abstract:
Abstract Biological invasions are a global threat to biodiversity, and many arise from deliberate introductions. The American freshwater fish Micropterus salmoides and Ameiurus spp. (Ameiurus melas and Ameiurus nebulosus) were introduced to Europe for recreational fishing, Gambusia holbrooki and Gambusia affinis were introduced for mosquito population control, and Lepomis gibbosus was introduced as an ornamental species. The Asiatic Pseudorasbora parva was acquired inadvertently as an accompanying species in fish consignments. This article presents a novel approach for detecting these species directly from water samples based on a panel of five taxonâspecific primers within 16S rDNA. The primers were validated from tissue, in aquarium experiments, and from Ebro River water samples (Spain). With a simple polymerase chain reaction (PCR) protocol, followed by visualization in agarose gel or capillary electrophoresis, it was possible to detect these species from environmental DNA concentrations as low as 0.89â100 pg mLâ1. This sensitive and economical tool can be used to control European invasions of these species and to preserve native biodiversity.
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689682
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