ID:
publications-1965
Type:
Peer reviewed articles
Year:
2022
Authors:
Alexandra Canciu; Andreea Cernat; Mihaela Tertis; Silvia Botarca; Madalina Adriana Bordea; Joseph Wang; Cecilia Cristea
Title:
Proof of Concept for the Detection with Custom Printed Electrodes of Enterobactin as a Marker of Escherichia coli
Venue/Journal:
International Journal of Molecular Sciences, Vol 23, Iss 17, p 9884 (2022)
DOI:
10.3390/ijms23179884
Research type:
Uncategorized
Water System:
Wastewater Treatment Plants
Technical Focus:
Abstract:
The rapid and decentralized detection of bacteria from biomedical, environmental, and food samples has the capacity to improve the conventional protocols and to change a predictable outcome. Identifying new markers and analysis methods represents an attractive strategy for the indirect but simpler and safer detection of pathogens that could replace existing methods. Enterobactin (Ent), a siderophore produced by Escherichia coli or other Gram-negative bacteria, was studied on different electrode materials to reveal its electrochemical fingerprint—very useful information towards the detection of the bacteria based on this analyte. The molecule was successfully identified in culture media samples and a future goal is the development of a rapid antibiogram. The presence of Ent was also assessed in wastewater and treated water samples collected from the municipal sewage treatment plant, groundwater, and tap water. Moreover, a custom configuration printed on a medical glove was employed to detect the target in the presence of another bacterial marker, namely pyocyanin (PyoC), that being a metabolite specific of another pathogen bacterium, namely Pseudomonas aeruginosa. Such new mobile and wearable platforms offer considerable promise for rapid low-cost on-site screening of bacterial contamination.
Link with Projects:
883484
Link with Tools:
Related policies:
ID:
